Autoclave PPT: Definition, Important Parts, Types, Uses
An autoclave is a machine that employs steam under pressure to kill bacteria, viruses, and even spores present in the material placed inside the vessel. The materials are sterilized in an autoclave by heating them to a predetermined temperature for a set amount of time. The autoclave, also known as a steam sterilizer, is a device that is extensively used in hospitals and companies for a variety of reasons. Because it uses moist heat sterilization, the autoclave is thought to be a more successful technique of sterilization.
Autoclave Important Parts:
- Chamber of Pressure: the pressure chamber, which is made up of an inner chamber and an outside jacket, is the most important part of a steam autoclave. The pressure chambers range in size from 100 to 3000 litres.
- Door/Lid: The lid’s job is to seal off the autoclave from the outside world and maintain a sterile environment inside. The screw clamps and asbestos washer keep the lid sealed.
- Electric heater/steam generator: Underneath the chamber is an electrical steam generator or boiler that employs an electric heating system to heat the water and generate steam in the inner and outer chambers.
- Cooler for wastewater: A mechanism to cool the effluent before it enters the drainage pipes is included in many autoclaves.
Types of Autoclave:
There are various types of autoclaves available on the market, including:
- Autoclaves of the sort used in pressure cookers/autoclaves used in laboratories (N-type)
- Autoclave with gravity displacement
- Type of positive pressure displacement (B-type)
- Type of negative pressure displacement (S-type)
Uses of Autoclave:
- They’re utilized to sterilize media, tools, and labware as well as decontaminate specific biological waste.
- Before being disposed of, medical waste that may contain bacteria, viruses, or other biological materials should be autoclaved to kill any bacteria, viruses, or other biological materials.